Developmental validation of VeriFiler™ Plus PCR Amplification Kit: A 6-dye multiplex assay designed for casework samples

Development and validation of the AmpFlSTR Yfiler PCR amplification kit: a male specific, single amplification 17 Y-STR multiplex system.

In the past 5 years, there has been a substantial increase in the use of Y-short tandem repeat loci (Y-STRs) in forensic laboratories, especially in cases where typing autosomal STRs has met with limited success. The AmpFlSTR Yfiler PCR amplification kit simultaneously amplifies 17 Y-STR loci including the loci in the "European minimal haplotype" (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DY...

Developmental validation of DogFiler, a novel multiplex for canine DNA profiling in forensic casework.

While the analysis of human DNA has been the focus of large-scale collaborative endeavors, non-human forensic DNA analysis has not benefited from the same funding streams and coordination of effort. Consequently, the development of standard marker panels, allelic ladders and allele-specific sequence data comparable to those established for human forensic genetics has lagged. To meet that need f...

Internal validation of the AmpFlSTR Yfiler amplification kit for use in forensic casework.

Y-chromosomal short-tandem repeat (Y-STR) amplification has been used in forensic casework at the Bureau of Criminal Apprehension (BCA) Forensic Science Laboratory since 2003. At that time, two separate amplifications were required to type the SWGDAM recommended loci (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS438, and DYS439). The Yfiler kit coamplifies these loci ...

Validation of a multiplex PCR assay for the simultaneous detection of human papillomavirus and Chlamydia trachomatis in cervical PreservCyt samples.

Validation of a Multiplex PCR Assay for the Simultaneous Detection of Human Papillomavirus and Chlamydia trachomatis in Cervical PreservCyt Samples

(MALDI)-TOF MS, with its higher resolution, is the capability of SELDI to enrich low-abundance proteins from complex matrices such as plasma, through, for example, the coupling of specific antibodies to the chip surfaces (4 ). Such an approach is an important methodologic aspect in “second phase” proteomics, which are characterized by repetitive investigation of the same protein to validate the...